Journal: International Journal of Nanomedicine

Article Title: Photothermal exposure of polydopamine-coated branched Au–Ag nanoparticles induces cell cycle arrest, apoptosis, and autophagy in human bladder cancer cells

doi: 10.2147/IJN.S174349

Figure Lengend Snippet: Changes of ΔΨm and the release of cytochrome c in T24 cells treated with different concentrations of Au–Ag@PDA NPs at 3 hours after laser irradiation with 1 W/cm 2 for 4 minutes. Notes: ( A ) The ΔΨm of T24 cells was determined by FCM using JC-1 staining. ( B ) Red/green fluorescence intensity ratios. ( C ) Level of cytosolic cytochrome c measured by Western blot. GAPDH was used as the loading control. (0-: 0 µg+no laser, 0: 0 µg+laser, 20: 20 µg+laser, 30: 30 µg+laser, 40: 40 µg+laser). ( D ) Quantification analysis of cytochrome c /GAPDH compared with the 0- control group (mean±SD; n=3). * P <0.05 vs 0 group. Abbreviations: ΔΨm, mitochondrial membrane potential; FCM, flow cytometry; NPs, nanoparticles; PDA, polydopamine.

Article Snippet: In brief, the cells were trypsinized, incubated with JC-1 solution at 37°C for 20 minutes, washed twice with PBS, and then analyzed using FCM (FACSCanto II; Becton, Dickinson and Company, Franklin Lakes, NJ, USA).

Techniques: Irradiation, Staining, Fluorescence, Western Blot, Flow Cytometry

Journal: CNS Neuroscience & Therapeutics

Article Title: Neuroprotective Effects of Transcranial Pulsed Current Stimulation: Modulation of Microglial Polarization in Traumatic Brain Injury

doi: 10.1111/cns.70606

Figure Lengend Snippet: Protective effects of tPCS on neurological function, pathological alterations, and inflammatory response following TBI. (A) Experimental procedure timeline. (B) Neurological function in mice was assessed using the mNSS at 1, 3, 5, and 7 dpo ( n = 8). (C) Representative exploration routes in the Y maze. (D) Time spent in the novel arm of the Y‐maze ( n = 8). (E) Beam‐balance test measuring the time required to traverse the beam ( n = 8). (F) Rotarod test measuring the latency to fall ( n = 8). (G) Representative scanning images and photomicrographs of H&E‐stained sections at 7 dpo ( n = 4). Arrows point to the neuronal edema, vacuolation, and degeneration. (H) Representative scanning images and photomicrographs of Nissl‐stained sections at 7 dpo ( n = 4). Arrows highlight neuronal vacuolation and degeneration. (G) Quantification of Nissl‐stained cells. (I–K) Quantitative analysis of Nissl‐stained cells, lesion volume, and correlation analysis between lesion volume and mNSS. (L) Representative images of TUNEL staining at 7 dpo. (M) Quantification of TUNEL‐stained cells ( n = 4). (N–P) The levels of inflammatory mediators including IL‐1β, TNF‐α, and IL‐10 in the peri‐lesional cortex were detected by ELISA ( n = 4). Scale bar = 100 μm. All values are mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: After dehydration and dewaxing, sections were incubated with TUNEL working solution (Servicebio, China) at 37°C for 2 h, followed by DAPI staining at 4°C for 8 min. After thorough rinsing, the sections were dried, mounted, and examined under a fluorescence microscope.

Techniques: Staining, TUNEL Assay, Enzyme-linked Immunosorbent Assay

Journal: Cell Communication and Signaling : CCS

Article Title: Atorvastatin promotes lipid catabolism in colorectal cancer via FDFT1‒mediated inhibition of the PI3K/AKT pathway

doi: 10.1186/s12964-026-02802-6

Figure Lengend Snippet: ATST induces ER stress and promotes apoptosis in CRC cells A Expression of ER stress‒related proteins in HCT116 cells after ATST treatment B Changes in intracellular Ca 2+ levels in HCT116 cells C Alterations in XBP‒1s mRNA expression in HCT116 cells D Representative IF images of XBP‒1s in HCT116 cells after ATST treatment. Scale bar: 50 μm E Apoptosis rate in HCT116 cells after ATST treatment F Changes in JC‒1 ratio in HCT116 cells following ATST treatment G Expression of apoptosis‒related proteins in HCT116 cells after ATST treatment H Representative IF images of CHOP in HCT116 cells after ATST treatment. Scale bar: 50 μm I Expression of SQSTM1 and LC3 A/B proteins in HCT116 cells after ATST treatment J Change in ROS levels in HCT116 cells K Cell cycle distribution in HCT116 cells after ATST treatment

Article Snippet: Cells were seeded in 6‒well plates at a density of 5 × 105 cells per well and treated with ATST at specified concentrations for 48 h. Cells were then collected and incubated with JC‒1 working solution (Yesen, 717164ES03, Shanghai) at 37 °C for 20 min. After washing twice with serum‒containing medium, samples were analysed by flow cytometry.

Techniques: Expressing